Programme

 

Mar
18
Sun
Opening Ceremony
Mar 18 @ 4:00 pm – 4:30 pm
Natarajan-memorial presentation
Mar 18 @ 4:30 pm – 5:00 pm
  • Margareta Törnqvist
GUM Award 2018 & Fritz Sobels Award
Mar 18 @ 5:00 pm – 7:00 pm
Welcome Reception
Mar 18 @ 7:00 pm – 8:00 pm
Mar
19
Mon
Registration
Mar 19 @ 8:00 am – 8:45 am
Plenary talk (Room 1)
Mar 19 @ 8:45 am – 9:30 am
  • Eugenia Dogliotti
    The cellular response to genotoxic DNA damage: the role of cell type and status.
Session I (Room 1) CELL TYPE SPECIFITICY OF GENOTOXICITY; DNA DAMAGE RESPONSE AND DNA REPAIR (Part 1)
Mar 19 @ 9:30 am – 10:30 am
  • Bernd Kaina
    DNA repair and death regulation in cells of the immune system
  • Jean-Marc Egly
    A new function for CSA and CSB in an ubiquitin-proteasome process required for transcription coupled repair and cellular division?
Session II (Room 2) Latest update of the GUM WORKING GROUPS, IWGT and the hComet project (Part 1)
Mar 19 @ 9:30 am – 10:30 am
  • Christina Ziemann, Bernd-Wolfgang Igl
    GUM AG Statistics, an interdisciplinary working group to bridge the gap between genetic toxicology and statistics
  • Andreas Zeller
    A critical appraisal of the sensitivity of in vivo genotoxicity assays in detecting human carcinogens
Coffee Break
Mar 19 @ 10:30 am – 11:00 am
Session I (Room 1) CELL TYPE SPECIFICITY OF GENOTOXICITY; DNA DAMAGE RESPONSE AND DNA REPAIR (Part 2)
Mar 19 @ 11:00 am – 12:30 pm
  • Maria Dusinska
    Genotoxicity of nanomaterials in advanced in vitro model systems
  • Speakers selected from abstracts: 4 x 15 min
    • Carina Ladeira
      Genotoxicity of Bisphenol A and Interference with Doxorubicin Effects in HEp-2 and MRC-5 Cell Lines
    • Wynand Roos
      Tolerance of genotoxic O6-methylguanine is caused by HDAC1/HDAC2/HDAC3 mediated RAD51 and FANCD2 expression in melanoma cells
    • Nadejda Rechkunova
      Nucleotide excision repair proteins and PARP1/PAR interplay: possible mechanism to regulate protein assembly on the damaged DNA
    • Andriy Khobta
      Fate of abasic sites resistant to base excision repair
Session II (Room 2) Latest update of the GUM WORKING GROUPS, IWGT and the hComet project (Part 2)
Mar 19 @ 11:00 am – 12:30 pm
  • Helga Stopper
    Fate of micronuclei and micronucleated cells – results of a GUM working group
  • Hans-Jörg Martus
    Feedback from 7th IWGT in Tokyo
  • Sabine Langie
    Identification of knowledge gaps and opportunities in the validation of comet assay based DNA repair phenotyping in human biomonitoring studies.
Lunch
Mar 19 @ 12:30 pm – 1:30 pm
Session III (Room 1) DISCOVERING CAUSES OF DISEASE: NEW APPROACHES IN HUMAN BIOMONITORING (Part 1)
Mar 19 @ 1:30 pm – 3:00 pm
  • Alexander Bürkle
    Biomarkers of human ageing – The EU FP7 MARK-AGE Project and beyond
  • Augustin Scalbert
    Recent progress in the measurement of the exposome through metabolomics
  • Speakers selected from abstracts: 2 x 15 min
    • Bernhard Monien
      Exposure to Glycidyl Ester-rich Vegetable Fat leads to Formation of N-(2,3-Dihydroxypropyl)-Valine in Hemoglobin: A Human Intervention Study
    • Kai Nieschalke
      Tryptophan adducts in blood proteins as novel biomarkers of exposure toward active metabolites of the rodent carcinogen methyleugenol

Session IV (Room 2) RISK ASSESSMENT OF GENETIC TOXICITY (Part 1)
Mar 19 @ 1:30 pm – 3:00 pm
  • David Kirkland
    An approach to weight of evidence assessment of genotoxicity data
  • Andreas Zeller
    Lower limits of critical effect sizes for the benchmark dose approach to assess dose–response relationships in genetic toxicology
  • Speakers selected from abstracts: 2 x 15 min
    • Steven Bryce
      Clastogenesis caused by primary versus secondary DNA damage distinguished by kinetics of γH2AX and phospho-histone H3 responses
    • Ryan Wheeldon
      Application of the BMD approach to quantitative multiplexed genetic toxicology data to derive compound potency ranking for several Topoisomerase II inhibitors
Coffee Break
Mar 19 @ 3:00 pm – 3:30 pm
Session III (Room 1) DISCOVERING CAUSES OF DISEASE: NEW APPROACHES IN HUMAN BIOMONITORING (Part 2)
Mar 19 @ 3:30 pm – 5:00 pm
  • Margareta Törnqvist
    Detection of internal exposure to toxic electrophiles in humans – achievements by hemoglobin adductomics
  • Silvia Balbo
    High resolution mass spectrometry-based approaches for the investigation of carcinogenesis
  • Speakers selected from abstracts: 2 x 15 min
    • Tabea Zubel
      A mass spectrometric platform for the quantitation of mustard-induced nucleic acid damage
    • Ezgi Eylül Bankoglu
      The effect of weight loss on antioxidant status and DNA damage in obese subjects
Session IV (Room 2) RISK ASSESSMENT OF GENETIC TOXICITY (Part 2)
Mar 19 @ 3:30 pm – 5:00 pm
  • Andrea Hartwig
    Risk assessment of non-genotoxic and genotoxic carcinogens: current approaches
  • Speakers selected from abstracts: 2 x 30 min
    • Kerstin Reisinger
      Use of newly validated in vitro genotoxicity assays support safety assessments of cosmetic ingredients
    • Rhiannon David
      The Next Generation of Genetic Toxicity Testing
Poster Session 1
Mar 19 @ 5:00 pm – 7:00 pm
GUM Assembly
Mar 19 @ 7:00 pm – 8:00 pm
Mar
20
Tue
Plenary talk (Room 1)
Mar 20 @ 8:45 am – 9:30 am
  • Vilhelm Bohr
    Signaling from Nuclear DNA damage to Mitochondrial Dysfunction
Session V (Room 1) DNA REPAIR AND DAMAGE RESPONSE (Part 1)
Mar 20 @ 9:30 am – 10:30 am
  • Nicolas Hoch
    DNA strand break repair and human disease
  • Gianluca Tell
    Non canonical roles of BER enzymes in RNA processing: novel perspectives in cancer biology
Session VI (Room 2) GENOME SAFETY IN GENE THERAPY (Part 1)
Mar 20 @ 9:30 am – 10:30 am
  • Silvana Libertini
    Safety of Advanced Therapies: Overview and challenges
  • Mike Boxem
    CRISPR/Cas9-engineering from a C. elegans perspective
Coffee Break
Mar 20 @ 10:30 am – 11:00 am
Session V (Room 1) DNA REPAIR AND DAMAGE RESPONSE (Part 2)
Mar 20 @ 11:00 am – 12:15 pm
  • Thomas Hofmann
    Cell fate decision-making by the cellular DNA damage response
  • Speakers selected from abstracts: 3 x 15 min
    • Natalia Lukianchikova
      Removal efficiency of DNA damages, forming a cluster, is defined by its mutual location
    • Theresa Maier
      Evaluation of in vitro DNA damage detection methods for the assessment of genotoxic and epigenetic biomarkers
    • Nadin Wedler
      Impact of zinc chloride, PJ-34, sodium arsenite and cobalt chloride on poly(ADP-ribosyl)ation and DNA single-strand break repair
Session VI (Room 2) GENOME SAFETY IN GENE THERAPY (Part 2)
Mar 20 @ 11:00 am – 12:15 pm
  • Manfred Schmidt
    Vector Persistence and Safety Considerations in Gene Therapy
  • Axel Schambach
    State-of-the-art assay systems to assess genotoxicity of integrating vectors
  • Speakers selected from abstracts: 1 x 15 min
    • Myriam Lemmens
      Development of an in vitro human cell transformation assay in order to evaluate the carcinogenicity of CRISPR/Cas9 based genome editing
Lunch
Mar 20 @ 12:15 pm – 1:15 pm
EEMGS Young Scientist 2018
Mar 20 @ 1:15 pm – 1:45 pm
EEMGS Assembly
Mar 20 @ 1:45 pm – 2:30 pm
Coffee
Mar 20 @ 2:30 pm – 3:00 pm
Session VII (Room 1) GENOTOXICITY TESTING
Mar 20 @ 3:00 pm – 3:45 pm
  • Speakers selected from abstracts: 3 x 15 min
    • Benjamin Kopp
      Combined Genotoxic Effects of Food Contaminant Mixtures in Liver cells
    • Maria João Silva
      Contribution to the hazard assessment of benchmark metallic nanomaterials through a set of in vitro genotoxicity assays
    • Nicola Winkelbeiner
      Investigating the role of base excision repair in the aging process assessing DNA incision activity via a non-radioactive cleavage assay
Session IX (Room 2) MUTANGENICITY AND AGING
Mar 20 @ 3:01 pm – 5:15 pm
  • Björn Schumacher
    DNA damage responses in aging and disease
  • Martijn Dollé
    Health and lifespan interventions in DNA repair deficient progeroid mice
  • Elżbieta Speina
    Lipid peroxidation is an endogenous factor of accelerated aging and death of ERCC1-deficient mice
  • Olga Lavrik
    The elevated activity of DNA repair systems of Heterocephalus glaber as possible basis of longevity
  • Speakers selected from abstracts: 1 x 15 min
    • Nicole Schupp
      The blood pressure regulating hormone angiotensin II causes an increased mutation frequency in the rat kidney
Session VIII (Room 1) ENVIRONMENTAL AND OCCUPATIONAL HEALTH
Mar 20 @ 3:45 pm – 5:15 pm
  • Lisbeth Knudsen
    Genotoxicity and human risk in environmental and occupational health
  • Speakers selected from abstracts: 4 x 15 min
    • Matthias  Hufnagel
      Elucidating the toxicological potential of nanoparticles using air-liquid interface exposure
    • Goran Gajski
      The comet assay as a reliable tool for the assessment of DNA damage in children exposed to diagnostic X-ray procedures
    • Mateja Sborchia
      The impact of p53 status on aristolochic acid I-induced nephrotoxicity, metabolism and DNA damage in Trp53(+/+), Trp53(+/-) and Trp53(-/-) mice
    • Heinke Bastek
      Aristolochic acid I DNA adduct formation in human renal cell systems
Poster Session 2
Mar 20 @ 5:15 pm – 7:00 pm
Conference Dinner
Mar 20 @ 7:15 pm – 11:00 pm
Mar
21
Wed
Plenary talk (Room 1)
Mar 21 @ 8:45 am – 9:30 am
  • Anton Gartner
    Massive C. elegans next generation sequencing to detect and understand mutational signatures
Session X (Room 1) ALTERNATIVES TO ANIMAL TESTING: EMERGING MODELS AND TECHNIQUES (Part 1)
Mar 21 @ 9:30 am – 10:30 am
  • George Johnson
    Developments in in vitro Pig-a
  • Thierry Durand
    Isoprostanoids, non-enzymatic oxidized metabolites of PUFAs: promising biomarkers in human, nuts, seeds, oils
Session XI (Room 2) UNDERSTANDING MECHANISM UNDERLYING MUTATIONAL SIGNATURES IN HUMAN CANCER USING WHOLE GENOME SEQUENCING (Part 1)
Mar 21 @ 9:30 am – 10:30 am
  • Jiri Zavadil
    Deciphering global mutation spectra of cancers using experimental systems
  • Volker M. Arlt
    Modelling mutational signatures of human carcinogens in cultured cells in vitro
Coffee
Mar 21 @ 10:30 am – 11:00 am
Session X (Room 1) ALTERNATIVES TO ANIMAL TESTING: EMERGING MODELS AND TECHNIQUES (Part 2)
Mar 21 @ 11:00 am – 12:15 pm
  • Speakers selected from abstracts: 5 x 15 min
    • Mustafa Abbass
      Genotoxicity assessment of benzo[a]pyrene in the invertebrate model Caenorhabditis elegans
    • Marc Audebert
      Screening of the genotoxic potential of 160 mycotoxins in human cell lines
    • Lisa Lemoine
      Microbially competent skin – a new skin model for testing genotoxicity in situ
    • Barbara Witt
      In vitro toxicological characterization of arsenolipids using selected neuronal in vitro models
    • Awadhesh Jha
      ‘Virtual fish’ tales: Liver, Intestinal and Gill Organoids as an in vitro alternative to live fish studies
Session XI (Room 2) UNDERSTANDING MECHANISM UNDERLYING MUTATIONAL SIGNATURES IN HUMAN CANCER USING WHOLE GENOME SEQUENCING (Part 2)
Mar 21 @ 11:00 am – 12:15 pm
  • Serena Nik-Zainal
    Utilising mutational signatures for clinical purposes
  • Moritz Gerstung
    Mutational signatures in C. elegans and human cancers
  • Speakers selected from abstracts: 1 x 15 min
    • Lisa Hölzl-Armstrong
      TP53 mutagenesis induced by acrylamide and glycidamide in human TP53 knock-in Hupki mouse embryo fibroblasts
Lunch
Mar 21 @ 12:15 pm – 1:30 pm
Session XII (Room 1) HOT TOPICS IN CARCINOGENICITY  (NON-DIRECT GENOTOXIC CARCINOGENS, EPIGENETICS AND GENOTOXICITY, DNA REPAIR)
Mar 21 @ 1:30 pm – 3:30 pm
  • Jörg Fahrer
    DNA damage response and DNA repair in colorectal cancer
  • Shana J. Sturla
    DNA Adduct Molecular Probes: From mechanisms of mutagenesis to adduct amplification
  • Monika Sramkova
    The Risks and Benefits of Nanotechnologies. Genetic and Epigenetic Toxicity
  • Speakers selected from abstracts: 2 x 15 min
    • Christina Hartmann
      Chronically increased concentrations of AngII cause impaired renal function, DNA double strand breaks and an initiation of epithelial to mesenchymal transition in rat kidneys
    • Daniel Heylmann
      Sensitivity of CD3/CD28-activated versus non-activated T lymphocytes towards ionizing radiation and genotoxic agents
Session XIII (Room 2) TESTING OF IMPURITY FOR MUTAGENIC POTENTIAL IN DRUGS, SUBSTANCES AND PRODUCTS
Mar 21 @ 1:30 pm – 3:30 pm
  • Roland Frötschl
    Current regulatory requirements for genotoxicity testing of impurities and metabolites in pharmaceuticals
  • Susanne Glowienke
    Experience with the ICH M7 class assignments
  • Jim Harvey
    Identification and management of potentially genotoxic and carcinogenic metabolites in drug discovery and development
  • Speakers selected from abstracts: 1 x 15 min
    • Maricel Marin-Kuan
      Improvement of genotoxicity assays limit of detection
Coffee break
Mar 21 @ 3:30 pm – 4:00 pm
Recent Insights in Regulatory Toxicology
Mar 21 @ 4:00 pm – 4:30 pm
  • Lutz Müller
    Accidental exposure of patients to high levels of mutagenic impurities in a drug – dose-response and risk assessment then and now
GUM Young Scientist 2018
Mar 21 @ 4:30 pm – 5:00 pm
Closing Remarks and Poster Awards
Mar 21 @ 5:00 pm – 5:30 pm
Mar
22
Thu
HESI GTTC Workshop: Applied Genetic Toxicity for Regulatory Decision Making: The Road Ahead
Mar 22 all-day

8AM – Room open for seating (Breakfast will not be provided)

Introduction – 8:30AM – 9:30 AM

8:30 – 9:00 AM Welcome and Workshop Objectives – Mirjam Luijten, RIVM, The Netherlands 

9:00 – 9:30 AM Overview of the GTTC – Structure, Purpose, and Recent Initiatives – Paul White, Health Canada, Canada 


Session I: Next Generation Assessment of Genomic Damage – “The Clean Sheet” 

9:30 -10:30 AM Path Forward for Genetic Toxicity Assessment – Kerry Dearfield, USDA (retired), USA

10:30 – 11:00 AM Break

11:00 – 12:00 PM The New Strategy in Practice: Case Study Examples – Veronique Thybaud, Sanofi, France & Nicholas Ball, Dow, Switzerland 

12:00 – 1:00 PM The Clean Sheet Strategy in Practice: Guided Discussion – Mirjam Luijten, RIVM, The Netherlands 

1:00 – 2:00 PM Lunch held in Mensa


Session II: Mode of Action Determination in Applied Genetic Toxicology 

2:00 – 2:30 PM Incorporating Mode of Action into Genetic Toxicity Assessment – Roland Frötschl, BfArM, Germany 

2:30 – 3:00 PM Novel Approaches and Technologies to Assess Genotoxic Modes of Action – Stefan Pfuhler, Procter & Gamble, USA 

3:00 – 3:30 PM Break

3:30 – 4:00 PM Genetic Toxicity Mini-Adverse Outcome Pathways (mini-AOPs) – Azeddine Elhajouji, Novartis, Switzerland 

4:00 – 5:00 PM Guided Discussion on Genotoxic Modes of Action – Maik Schuler, Pfizer, USA 

Mar
23
Fri
HESI GTTC Workshop: Applied Genetic Toxicity for Regulatory Decision Making: The Road Ahead
Mar 23 all-day

8:00 – 8:30 AM Room open for seating (breakfast will not be provided)

 

Session III Quantitative Approaches 

8:30 – 9:15 AM Approaches for Analyses and Interpretation of Genetic Toxicity Dose-Response Data – Paul White, Health Canada, Canada 

9:15 – 10:00 AM Quantitative Dose-Response Analyses for Risk Assessment and Regulatory Decision-Making: Issues, Applications, and Challenges- George Johnson, Swansea University, UK 

10:00 – 10:30 AM Break

10:30 – 11:30 AM Quantitative Dose-Response Analysis in Genetic Toxicology: Guided Discussion Paul White, Health Canada, Canada & George Johnson, Swansea University, UK 

11:30 – 11:45 AM Introduction of the Breakout Groups

11:45 – 12:30 PM Lunch held in Mensa

Session IV: The Road Ahead

12:30 – 2:00 PM Break out group discussions of GTTC case studies – Lead: Mirjam Luijten, RIVM, The Netherlands 

Plenary reporting and discussion on successes, opportunities, and challenges

2:00 – 3:00 PM The Road Ahead: a guided panel discussion with various stakeholders – Jan van Benthem, RIVM, The Netherlands & Marilyn Aardema, Marilyn Aardema Consulting, USA

3:00 PM Adjourn

An agenda for the HESI GGTC workshop that follows the EEMGS meeting is available here